![]() ![]() Surgery combined with concurrent chemo-radiotherapy can cure 80%–95% early cervical cancer patients, but the treatment effect is not satisfactory for advanced and metastatic cervical cancers. The therapeutic methods for cervical cancer include surgery, medication, radiotherapy, heat treatment and gene therapy. Therefore, F2 and F5 could target the JAK2 protein and prevent the phosphorylation of JAKs to suppress the phosphorylation of the downstream effector Stats, which suggested that F2 and F5 have great potential to be the inhibitors of the JAK/Stat signaling pathway.Ĭervical carcinoma is the second most common malignancy among women in the world and the age of onset is declining recent years. Moreover, F2 and F5 showed the same downregulation on the P-Stat3 (Tyr705). While F5 could increase the expression of JAK2 and naturally decrease the phosphorylation of JAK1 and Tyk2, and decreased the expression of P-Stat6. F2 increased the expression of JAK2 and downregulated the level of P-JAK1 and P-JAK2, and decreased P-Stat3 (Ser727). Interestingly, F2 and F5 showed diverse regulation for signaling molecules because of their different chemical structure. Western blot analysis, immunofluorescence staining study and PCR was conducted to investigate the molecules of JAK/Stat signaling pathway. And the analytical methods of surface plasmon resonance and quartz crystal microbalance were established and employed to study the interaction between F2 and F5 and potential target protein JAK2, suggesting that both compounds have strong interaction with the JAK2 protein. In this study, Real Time Cell Analysis and crystal violet staining assay were conducted to investigate the effect of F2 and F5 on HeLa cells proliferation. Our study aims to confirm the potential targets of F2 and F5, and investigate the underlying mechanism of the inhibitory effect on HeLa cells. TAD-1822-7-F2 (F2) and TAD-1822-7-F5 (F5) are novel compounds synthesized on the chemical structure of taspine derivatives, and show an effective suppression for HeLa cells. ![]() Cervical carcinoma remains the second most common malignancy with a high mortality rate among women worldwide. ![]()
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